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Our Abpromise guarantee covers the use of ab18318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). It is strongly recommended that immunoprecipitation with a pan-specific Aurora antibody be carried out before Western blotting with the phospho-specific antibody to increase signal. Weak non specific bands can be sometimes observed above the band of interrest. A large non specific band is also present just above 32kDa.|
|ICC/IF||1/500 - 1/1000.|
Prometaphase HeLa cells were permaeabiliased in 0.2% Triton-X100 and fixed for 10 minutes at room temperature in 4% formaldehyde. The cells were washed 3 times in PBS and incubated with ab18318 (1/1000) for 1 hour at room temperature. The panel of images shows the nuceli stained with DAPI (blue) and the centromeres stained with ab18318 (green). 100x magnification.
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