- 数据表
- 具体的参考文献 (5)
- 实验方案
概述
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产品名称Anti-Aurora A抗体- Centrosome Marker
参阅全部 Aurora A 一抗 -
描述兔多克隆抗体to Aurora A - Centrosome Marker
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宿主Rabbit
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特异性ab12875 detects a single clean band of just below 50kD in Jurkat and Hela cell lysates. This band corresponds to the size of Aurora A seen using other antibodies.
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经测试应用适用于: IHC-P, WB, ICC/IF, ICCmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aurora A.
(Peptide available as ab13008.)
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阳性对照
- Jurkat cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab12875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| WB | ||
| ICC/IF | ||
| ICC |
应用说明
ICC: 1/500.
IF: 1/500.
IHC-P: 1/15 - 1/20. Antigen retrieval was performed using either Sodium Citrate or Tris/EDTA before commencing with the IHC staining protocol. Customer feedback suggests that a 1/15 dilution of ab12875 is recommended when using the sodium citrate antigen retreival method and a 1/20 dilution is recommended when using the Tris/EDTA antigen retrieval method.
WB: 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IF: 1/500.
IHC-P: 1/15 - 1/20. Antigen retrieval was performed using either Sodium Citrate or Tris/EDTA before commencing with the IHC staining protocol. Customer feedback suggests that a 1/15 dilution of ab12875 is recommended when using the sodium citrate antigen retreival method and a 1/20 dilution is recommended when using the Tris/EDTA antigen retrieval method.
WB: 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
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组织特异性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
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序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
- Information by UniProt
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数据库链接
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
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别名
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
图片
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Western blot - Anti-Aurora A antibody - Centrosome Marker (ab12875)All lanes : Anti-Aurora A antibody - Centrosome Marker (ab12875) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor 680 Goat anti Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 48 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry - Anti-Aurora A antibody - Centrosome Marker (ab12875)This image was submitted as part of a review by Kirk McManus, University of British ColumbiaMetaphase HeLa cells stained with ab12875 and counterstained with DAPI. The antibody shows the localisation of Aurora A to the centrosome during metaphase. 100x magnification.
Panel 1: DNA stained with DAPI.
Panel 2: Cells stained with ab12875 (1/500 dilution).
Panel 3: DAPI staining is shown in red and ab12875 in green. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody - Centrosome Marker (ab12875)The image shows staining of formalin fixed paraffin embedded human tonsil sections with ab12875 (1/20 working dilution). Clear nuclear staining can be seen.
实验方案
文献
This product has been referenced in:
- Hammond D et al. Melanoma-associated mutations in protein phosphatase 6 cause chromosome instability and DNA damage owing to dysregulated Aurora-A. J Cell Sci 126:3429-40 (2013). Read more (PubMed: 23729733) »
- Zeng K et al. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol 191:1315-32 (2010). Read more (PubMed: 21187329) »
客户评价及客户问答
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