概述
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产品名称Anti-Aurora A抗体
参阅全部 Aurora A 一抗 -
描述兔多克隆抗体to Aurora A
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宿主Rabbit
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特异性ab61114 detects endogenous levels of total Aurora A protein.
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经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic non-phosphopeptide derived from human Aurora A around the phosphorylation site of threonine 288 (R-T-TP-L-C).
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阳性对照
- extracts from 293 cells treated with serum (20%, 15mins).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab61114 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab61114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 4 µg/ml. | |
| ICC/IF | 1/500. (see Abreview) | |
| ELISA | 1/5000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
靶标
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功能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
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组织特异性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
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序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
- Information by UniProt
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数据库链接
- Entrez Gene: 6790 Human
- Entrez Gene: 20878 Mouse
- Entrez Gene: 261730 Rat
- Omim: 603072 Human
- SwissProt: O14965 Human
- SwissProt: P97477 Mouse
- SwissProt: P59241 Rat
- Unigene: 250822 Human
see all -
别名
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
图片
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Western blot - Anti-Aurora A antibody (ab61114)All lanes : Anti-Aurora A antibody (ab61114) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated
with serum (20%, 15mins)
Lane 2 : Extracts from 293 cells treated
with serum (20%, 15mins) with immunising Aurora A peptide
Predicted band size: 46 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody (ab61114)ab61114 staining Aurora A in human ovarian carcinoma.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody (ab61114)This image is courtesy of an Abreview by Ioannis Gavvodisab61114 staining Aurora A in Human Fibroblast cells by Immunocytochemistry/Immunofluorescence. Cells were PFA-fixed and permeabilized in methanol for 30 minutes at -20°C prior to blocking in 20% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/500 with the blocking buffer and incubated with the sample for 1 hour. The secondary antibody was a Alexa Fluor 594® conjugated Goat anti-Mouse polyclonal, diluted 1/1000.
DAPI was used for the nuclear counterstain.
实验方案
文献
ab61114 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"