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Our Abpromise guarantee covers the use of ab1287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/1000 for 1 hr on HeLa cells (see Abreview).|
|IHC-P||1/250 - 1/1000.|
|WB||1/4000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast, pancreas and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.|
|IP||Use a concentration of 2 - 10 µg/ml.|