Anti-Aurora A抗体(ab12324)

概述

  • 产品名称Anti-Aurora A抗体
    参阅全部 Aurora A 一抗
  • 描述
    兔多克隆抗体to Aurora A
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Immunogen was a synthetic peptide, which represented a C-terminal domain of mouse Aurora Kinase A (LocusLink ID 20878).

  • 阳性对照
    • Rat mammary glands.

性能

应用

Our Abpromise guarantee covers the use of ab12324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/2000 - 1/15000. Predicted molecular weight: 48 kDa.

靶标

  • 功能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 组织特异性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A antibody 图像



  • Predicted band size : 48 kDa


    Detection of Rat Aurora A by Western Blot. Samples: Whole tissue lysate (60 mcg/lane) from rat mammary glands. Antibody: ab12324 used at 0.2 mcg/ml. Detection: Chemiluminescence with an exposure time of 6 minutes. *Note: The upper band is believed to be phosphorylated Aurora A.

Anti-Aurora A antibody (ab12324)参考文献

ab12324 has not yet been referenced specifically in any publications.

Product Wall

This antibody has not been tested in human, only rat and mouse, though there is good homology between the species (82%). I recommend to use the positive control of rat mammary gland lysate to check the antibody works/is not damaged.

Thank you for your enquiry regarding ab12324. This antibody was tested for Western blot application using rat whole lysate from mammary glands. As the Western blot image on our datasheet shows there is a distinct band at around 48 kDa. As we und...

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No, ab12324 has not been tested for cross-reactivity with Aurora B. If you hvae any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"