概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100 - 1/150. Primary: 1/1000 - 1/30000.
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.
IP Use at an assay dependent dilution.

靶标

  • 别名
    • AU1 epitope tag antibody
    • DTYRYI epitope tag antibody
    • DTYRYI tag antibody

图片

  • The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines. (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).

    Picture from review by Amith Panner, submitted 29 July 2004.

文献

This product has been referenced in:
  • Yang CS  et al. Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome. Nat Commun 6:6115 (2015). WB . Read more (PubMed: 25655831) »
  • Jones DT  et al. Recurrent somatic alterations of FGFR1 and NTRK2 in pilocytic astrocytoma. Nat Genet 45:927-32 (2013). Read more (PubMed: 23817572) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your enquiry and interest in our products.

This antibody has been tested in ICC so it is very likely to work in Confocal microscopy, though it has not been specifically characterized in this application.

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Je vous contacte suite aux questions auxquelles vous avez répondues il y a quelques jours concernant la qualité des produits et des services Abcam. Je vous remercie d'avoir pris le temps de répondre à ces questions.
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Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3401. L'immunogène ayant servi à produire cet anticorps est DTYRYI. Je ne pense pas que la présence de 2 résidus avant cett...

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The AU1-AU5 tags are epitope tags and are fairly uncommon. In the instance of ab3401, the sequence DTYRYI is the sequence of the tag. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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