Anti-ATXN2L抗体(ab99304)
Key features and details
- Rabbit polyclonal to ATXN2L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ATXN2L抗体
参阅全部 ATXN2L 一抗 -
描述
兔多克隆抗体to ATXN2L -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
Database link: NP_009176.2 -
阳性对照
- WB: HeLa and 293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99304于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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组织特异性
Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells. -
序列相似性
Belongs to the ataxin-2 family. -
翻译后修饰
Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
Arg-361 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11273 Human
- Entrez Gene: 233871 Mouse
- Entrez Gene: 361649 Rat
- Omim: 607931 Human
- SwissProt: Q8WWM7 Human
- SwissProt: Q7TQH0 Mouse
- Unigene: 460499 Human
- Unigene: 231450 Mouse
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别名
- A2D antibody
- A2LG antibody
- A2LP antibody
see all
图片
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All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds -
ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99304 尚未被引用在任何文献中。