概述

  • 产品名称Anti-ATTY抗体[EPR6121]
    参阅全部 ATTY 一抗
  • 描述
    兔单克隆抗体[EPR6121] to ATTY
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human ATTY.

  • 阳性对照
    • HepG2 (treated with insulin), HepG2, and A549 cell lysates; Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab125000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.
    • 通路Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.
    • 疾病相关Defects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.
    • 序列相似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATTY_HUMAN antibody
      • L-tyrosine:2-oxoglutarate aminotransferase antibody
      • TAT antibody
      • Tyrosine aminotransferase antibody
      • Tyrosine aminotransferase, cytosolic antibody
      see all

    Anti-ATTY antibody [EPR6121] 图像

    • All lanes : Anti-ATTY antibody [EPR6121] (ab125000) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate, treated with insulin
      Lane 2 : HepG2 cell lysate, untreated
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 50 kDa
    • ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ATTY antibody [EPR6121] (ab125000)参考文献

    This product has been referenced in:
    • Nandi SS  et al. Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes 65:3075-90 (2016). Read more (PubMed: 27411382) »

    See 1 Publication for this product

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