概述

  • 产品名称Anti-Atrophin 1抗体
    参阅全部 Atrophin 1 一抗
  • 描述
    兔多克隆抗体to Atrophin 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human Atrophin 1.

    (Peptide available as ab32861.)

  • 阳性对照
    • This antibody gave a positive signal in Human Testis Tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab32604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).

靶标

Anti-Atrophin 1 antibody 图像

  • IHC image of ab32604 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32604, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Anti-Atrophin 1 antibody (ab32604) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
    Additional bands at : 100 kDa,55 kDa,76 kDa. We are unsure as to the identity of these extra bands.

Anti-Atrophin 1 antibody (ab32604)参考文献

ab32604 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab32604.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"