The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: 1/100 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
序列相似性Belongs to the ATRIP family.
结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
翻译后修饰Phosphorylated by ATR.
细胞定位Nucleus. Redistributes to discrete nuclear foci upon DNA damage.