Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg +2 and Ca +2), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Belongs to the ATRIP family.
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Phosphorylated by ATR.
Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
Information by UniProt
ATM and Rad3 related interacting protein antibody
Anti-ATRIP antibody 图像
Immunohistochemistry (Paraffin-embedded sections) - ATRIP antibody (ab59346)
ab59346, at a 1/50 dilution, staining human ATRIP in lung carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
Western blot - Anti-ATRIP antibody (ab59346)
Anti-ATRIP antibody (ab59346) at 1/1000 dilution + Human mammary cell lysate at 50000 cells
Goat anti-rabbit HRP IgG at 1/2500 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Exposure time :
This image is courtesy of an anonymous Abreview
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