概述

  • 产品名称Anti-ATRIP抗体
    参阅全部 ATRIP 一抗
  • 描述
    兔多克隆抗体to ATRIP
  • 特异性ab59346 detects endogenous levels of total ATRIP protein.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human ATRIP around the phosphorylation site of serine 68/serine 72 (LASPQA).

  • 阳性对照
    • Human lung carcinoma tissue
  • 常规说明Store at -20°C for 1 year

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

靶标

  • 功能Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the ATRIP family.
  • 结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻译后修饰Phosphorylated by ATR.
  • 细胞定位Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGS1 antibody
    • ATIP antibody
    • ATM and Rad3 related interacting protein antibody
    • ATM and Rad3-related-interacting protein antibody
    • ATR interacting protein antibody
    • ATR-interacting protein antibody
    • Atrip antibody
    • ATRIP_HUMAN antibody
    • DKFZp762J2115 antibody
    • FLJ12343 antibody
    • MGC20625 antibody
    • MGC21482 antibody
    • MGC26740 antibody
    see all

Anti-ATRIP antibody 图像

  • ab59346, at a 1/50 dilution, staining human ATRIP in lung carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
  • Anti-ATRIP antibody (ab59346) at 1/1000 dilution + Human mammary cell lysate at 50000 cells

    Secondary
    Goat anti-rabbit HRP IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-ATRIP antibody (ab59346)参考文献

This product has been referenced in:
  • Neidhart M  et al. Trex-1 deficiency in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 62:2673-9 (2010). Flow Cyt ; Human . Read more (PubMed: 20496420) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cell)
Loading amount 50000 cells
Specification Human mammary cell
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"