产品名称 Anti-ATRIP抗体 参阅全部 ATRIP 一抗
特异性 ab59346 detects endogenous levels of total ATRIP protein.
IHC-P, ELISA, WB more details
Synthesized non phosphopeptide derived from human ATRIP around the phosphorylation site of serine 68/serine 72 (LAS
Human lung carcinoma tissue
常规说明 Store at -20°C for 1 year
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg +2 and Ca +2), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
功能 Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
序列相似性 Belongs to the ATRIP family.
结构域 The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
翻译后修饰 Phosphorylated by ATR.
细胞定位 Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
Information by UniProt
ATM and Rad3 related interacting protein antibody
Anti-ATRIP antibody 图像
Immunohistochemistry (Paraffin-embedded sections) - ATRIP antibody (ab59346)
ab59346, at a 1/50 dilution, staining human ATRIP in lung carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
Western blot - Anti-ATRIP antibody (ab59346)
Anti-ATRIP antibody (ab59346) at 1/1000 dilution + Human mammary cell lysate at 50000 cells Secondary Goat anti-rabbit HRP IgG at 1/2500 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 85 kDa Observed band size : 85 kDa Exposure time : 1 minute This image is courtesy of an anonymous Abreview
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