The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. Staining of the AtRabA4b band in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Rab GTPases belong to the superfamily of small GTP binding proteins that include Ras, Rho, Arf and Ran GTPases. The Rab GTPases are involved in the regulation of diverse eukaryotic cellular processes such as cytoskeletal assembly and organization, cellproliferation and intracellular membrane trafficking. In eukaryotic cells Rab GTPases regulate membrane trafficking
events associated with distinct compartments. The type of subcellular compartment with which Rab GTPase is associated can be predicted based on sequence similarity.
The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b is highly similar to AtRab11. AtRabA4b labels a novel compartment that accumulates at the tips of expanding root hair cells. It has been suggested that in A. thaliana, the RabA4b GTPase regulates membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b has been shown to label a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing A. thaliana root hair cells. GTP bound AtRabA4b selectively recruits the plant phosphatidylinositol-4- kinase PI-4Kb1, but not other members of PI-4K families.