概述

  • 产品名称Anti-ATR抗体
    参阅全部 ATR 一抗
  • 描述
    兔多克隆抗体to ATR
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Xenopus laevis
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human ATR and Rad3 related encoded within exon 29 (LocusLink ID 545).

  • 阳性对照
    • Whole cell lysate from HeLa and U2OS cells.

性能

应用

Our Abpromise guarantee covers the use of ab10327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 307 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • 组织特异性Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • 疾病相关Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • 翻译后修饰Phosphorylated; autophosphorylates in vitro.
    • 细胞定位Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all

    Anti-ATR antibody 图像

    • All lanes : Anti-ATR antibody (ab10327) at 0.07 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : U2OS whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : U2OS whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique

      Predicted band size : 307 kDa

    Anti-ATR antibody (ab10327)参考文献

    This product has been referenced in:
    • Wang WH  et al. Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283:25455-67 (2008). WB ; Mouse . Read more (PubMed: 18606816) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. We recommend a 3-8% Tris-acetate gel. I will add the image of the blot of ATR in HeLa and U2OS cells on our datasheet as soon as possible to show the bands expected, Please do not hesitate to contact us again if you need...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"