概述

  • 产品名称Anti-ATR抗体
    参阅全部 ATR 一抗
  • 描述
    兔多克隆抗体to ATR
  • 特异性We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Ataxia and Rad3 related encoded within exon 5 (LocusLink ID 545).

  • 阳性对照
    • Whole cell lysate from HeLa and U2OS cells

性能

应用

Our Abpromise guarantee covers the use of ab10312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 317 kDa). Block membrane with 5% milk and incubate antibodies (primary and secondary) in milk.
    This antibody can often recognise extra bands which are fragments of ATR.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • 组织特异性Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • 疾病相关Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • 翻译后修饰Phosphorylated; autophosphorylates in vitro.
    • 细胞定位Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all

    Anti-ATR antibody 图像



    • Predicted band size : 317 kDa


      Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.

    Anti-ATR antibody (ab10312)参考文献

    This product has been referenced in:
    • Zhang P  et al. ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol 16:864-75 (2014). Read more (PubMed: 25086746) »
    • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »

    See all 4 Publications for this product

    Product Wall

    Thank you for taking the time to fill out the technical questionaire and send it in to us. I apologise for the delay in getting back to you. However, I have more details of the western blot testing procedure for this antibody from the laboratory which ...

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    You recently enquired about our ATM and ATR antibodies and my colleague Karen has already sent you a reply regarding the antibodies ab2354 and ab4471. I have also investigated your enquiry regarding ab10312. The antibody should not react with ATM ow...

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