概述

  • 产品名称Anti-ATR抗体[2B5]
    参阅全部 ATR 一抗
  • 描述
    小鼠单克隆抗体[2B5] to ATR
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

  • 阳性对照
    • Raji cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab4471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20655466
WB 1/500 - 1/1000. Detects a band of approximately 317 kDa (predicted molecular weight: 317 kDa).

靶标

  • 功能Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • 组织特异性Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • 疾病相关Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰Phosphorylated; autophosphorylates in vitro.
  • 细胞定位Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all

Anti-ATR antibody [2B5] 图像



  • Predicted band size : 317 kDa


    Western blot analysis of ATR protein expression in human Raji whole cell extract.

Anti-ATR antibody [2B5] (ab4471)参考文献

This product has been referenced in:
  • Menon VR  et al. Ligand modulation of a dinuclear platinum compound leads to mechanistic differences in cell cycle progression and arrest. Biochem Pharmacol 86:1708-20 (2013). Human . Read more (PubMed: 24161784) »
  • Blackford AN  et al. Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation. Proc Natl Acad Sci U S A 107:12251-6 (2010). WB ; Human . Read more (PubMed: 20566845) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 15 2007

I'm very sorry to hear the recent experiments did not give you satisfactory results. I have arranged for the replacement vial to be sent to you, on order 189135 (a confirmation fax has been sent to this number: 01214158781); we have some in stock (...

Read More

My sincere apologies for the delay in resolving this matter, I have been ill the last few days and was still waiting to hear back from the source of ab4471 for some feedback. They replied to me the following comments: "The only suggestions that I could...

Read More

Thank you for your e-mail and your order details. I have arranged for a replacement vial to be sent to you; this is placed on order 161886 and should be dispatched today. Could you please let me know if you are satisfied with the replacement vial?

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"