概述

  • 产品名称
  • 描述
    鸡多克隆抗体to ATPB
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    The immunogen corresponds to C terminal amino acids 291-539 of Human Mitochondrial ATP Synthase Subunit Beta (ATPB).

性能

应用

Our Abpromise guarantee covers the use of ab37922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 57 kDa.

靶标

  • 功能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 序列相似性
    Belongs to the ATPase alpha/beta chains family.
  • 细胞定位
    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

图片

  • Anti-ATPB antibody (ab37922) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg

    Secondary
    Goat anti-IgY-HRP at 1/1000 dilution

    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab37922 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry
Sample
Human Cultured Cells (Podocytes)
Specification
Podocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
Username

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提交于 May 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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