Anti-ATPB抗体[3D5] - Mitochondrial Marker (ab14730)

概述

性能

应用

Our Abpromise guarantee covers the use of ab14730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-Fr 1/100.
ICC/IF Use a concentration of 1 - 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 21490313
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 序列相似性
    Belongs to the ATPase alpha/beta chains family.
  • 细胞定位
    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Anti-ATPB antibody [3D5] - Mitochondrial Marker 图像

  • ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 µg/ml)
  • All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    Lane 1 : isolated mitochondria from human heart at 5 µg
    Lane 2 : isolated mitochondria from bovine heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab14730 staining ATPB in rat liver tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 18 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

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  • ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab14730 (blue) at 1µg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.
  • All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)参考文献

This product has been referenced in:
  • Rohlenova K  et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2(high) Breast Cancer. Antioxid Redox Signal 26:84-103 (2017). Read more (PubMed: 27392540) »
  • Matsuhashi T  et al. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases. EBioMedicine 20:27-38 (2017). WB ; Cow . Read more (PubMed: 28579242) »

See all 69 Publications for this product

Product Wall

Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (20ul of 60ul total protein extract from 4 whole fl)
Gel Running Conditions
Reduced Denaturing (4-12% BIS-TRIS PLUS)
Loading amount
1.3 cells
Specification
20ul of 60ul total protein extract from 4 whole fl
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (human fibroblast cell and mitochondria fraction)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human fibroblast cell and mitochondria fraction
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat primary fibroblasts)
Permeabilization
Yes - 0.5% TX100
Specification
Rat primary fibroblasts
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Nov 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human primary fibroblasts)
Permeabilization
Yes - 0.5% TX100
Specification
human primary fibroblasts
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Oct 10 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Human Cell (MDA MB 231 cells)
Specification
MDA MB 231 cells
Permeabilization
Yes - 1% Triton
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Dog Cell (MDCK2 cells)
Specification
MDCK2 cells
Permeabilization
Yes - 0.5% TX100
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Feb 09 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Pig Cell lysate - whole cell (porcine retinal pigment epithelium (RPE) cells)
Specification
porcine retinal pigment epithelium (RPE) cells
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jan 30 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (human primary fibroblasts)
Specification
human primary fibroblasts
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jan 29 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Dog Cell lysate - whole cell (MDCK2 cells)
Specification
MDCK2 cells
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jan 21 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (mouse astrocytes)
Specification
mouse astrocytes
Permeabilization
Yes - 0.5% TX100
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jan 13 2015

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