Anti-ATPase Inhibitory Factor 1/IF1抗体[5E2D7] (ab110277)
Key features and details
- Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1
- Suitable for: ICC/IF, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-ATPase Inhibitory Factor 1/IF1抗体[5E2D7]
参阅全部 ATPase Inhibitory Factor 1/IF1 一抗 -
描述
小鼠单克隆抗体[5E2D7] to ATPase Inhibitory Factor 1/IF1 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Isolated mitochondria from Human heart, Bovine heart, Rat heart and Mouse heart IF/ICC: HepG2 cell line.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
The purity of ab110277 is near homogeneity, as judged by SDS-PAGE. ab110277 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
5E2D7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 10,18 kDa (predicted molecular weight: 12 kDa).
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 10,18 kDa (predicted molecular weight: 12 kDa). |
靶标
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功能
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. -
序列相似性
Belongs to the ATPase inhibitor family. -
翻译后修饰
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 327699 Cow
- Entrez Gene: 93974 Human
- Entrez Gene: 11983 Mouse
- Entrez Gene: 25392 Rat
- SwissProt: P01096 Cow
- SwissProt: Q9UII2 Human
- SwissProt: O35143 Mouse
- SwissProt: Q03344 Rat
see all -
别名
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
see all
图片
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Immunocytochemistry analysis of paraformaldehyde-fixed rat primary cardiac fibroblasts permeabilized with 1% Triton for 4 min staining with ab110277 at 1/50 dilution. Secondary antibody was Dylight™ 488 donkey anti mouse at 1/100 dilution. Samples were incubated with the primary antibody with PBST for 30 minutes at 25°C
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All lanes : Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ATPase Inhibitory Factor 1 / IF1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 12 kDaLanes 1 - 3: Merged signal (red and green). Green - ab110277 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab110277 was shown to recognize ATPase Inhibitory Factor 1 / IF1 in wild-type HAP1 cells as signal was lost at the expected MW in ATPase Inhibitory Factor 1 / IF1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ATPase Inhibitory Factor 1 / IF1 knockout samples were subjected to SDS-PAGE. Ab110277 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
ICC/IF image of ab110277 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110277, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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All lanes : Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277) at 1 µg/ml
Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Predicted band size: 12 kDa -
Overlay histogram showing HepG2 cells stained with ab110277 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110277, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab91353) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab110277 被引用在 24 文献中.
- Zhang K et al. ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis. Lab Invest 102:69-79 (2022). PubMed: 34608240
- Ma KY et al. Parkinson's disease-associated VPS35 mutant reduces mitochondrial membrane potential and impairs PINK1/Parkin-mediated mitophagy. Transl Neurodegener 10:19 (2021). PubMed: 34127073
- Wang Y et al. Mitochondrial protein IF1 is a potential regulator of glucagon-like peptide (GLP-1) secretion function of the mouse intestine. Acta Pharm Sin B 11:1568-1577 (2021). PubMed: 34221868
- Kahancová A et al. Overexpression of native IF1 downregulates glucose-stimulated insulin secretion by pancreatic INS-1E cells. Sci Rep 10:1551 (2020). PubMed: 32005857
- Anand R et al. MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. Life Sci Alliance 3:N/A (2020). PubMed: 32788226