概述

  • 产品名称Anti-ATP7b抗体[EPR6794]
    参阅全部 ATP7b 一抗
  • 描述
    兔单克隆抗体[EPR6794] to ATP7b
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of the Human ATP7B protein (P35670).

  • 阳性对照
    • Caco-2 and HepG2 cell lysates and HepG2 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 157 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
    • 组织特异性Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
    • 疾病相关Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
    • 序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
      Contains 6 HMA domains.
    • 翻译后修饰Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
    • 细胞定位Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP7B antibody
      • ATP7B_HUMAN antibody
      • ATPase, Cu(2+) transporting, beta polypeptide antibody
      • ATPase, Cu++ transporting, beta polypeptide antibody
      • Copper pump 2 antibody
      • Copper transporting ATPase 2 antibody
      • PWD antibody
      • Toxic milk antibody
      • tx antibody
      • WC1 antibody
      • WD antibody
      • Wilson disease associated protein antibody
      • Wilson disease-associated protein antibody
      • WND antibody
      • WND/140 kDa antibody
      see all

    Anti-ATP7b antibody [EPR6794] 图像

    • All lanes : Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 157 kDa
    • Flow cytometric analysis of permeabilized HepG2 cells using anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ATP7b antibody [EPR6794] (ab124973)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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