概述

  • 产品名称Anti-ATP6V1E1抗体
    参阅全部 ATP6V1E1 一抗
  • 描述
    兔多克隆抗体to ATP6V1E1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 160 of Human ATP6V1E1 (UniProt ID: P36543).

  • 阳性对照
    • A549 and Mouse brain whole cell lysates; HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab111733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100 - 1/500.

靶标

  • 相关性ATP6V1E1 is a component of vacuolar ATPase (V ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This protein is alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
  • 细胞定位Plasma membrane
  • 数据库链接
  • 别名
    • ATP6E antibody
    • ATP6E2 antibody
    • ATP6V1E antibody
    • ATPase H+ transporting lysosomal 31kDa V1 subunit E1 antibody
    • ATPase H+ transporting V1 subunit E1 antibody
    • H(+) transporting two sector ATPase 31kDa subunit antibody
    • H+ transporting ATP synthase chain E vacuolar antibody
    • P31 antibody
    • V ATPase 31 kDa subunit antibody
    • V ATPase subunit E 1 antibody
    • V ATPase subunit E antibody
    • V-type proton ATPase subunit E 1 antibody
    • Vacuolar proton pump subunit E 1 antibody
    • Vma4 antibody
    see all

Anti-ATP6V1E1 antibody 图像

  • Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 26 kDa
  • Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size : 26 kDa
  • Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.

Anti-ATP6V1E1 antibody (ab111733)参考文献

This product has been referenced in:
  • Cao X  et al. V-ATPase promotes transforming growth factor-ß-induced epithelial-mesenchymal transition of rat proximal tubular epithelial cells. Am J Physiol Renal Physiol 302:F1121-32 (2012). WB, ICC/IF ; Rat . Read more (PubMed: 22129967) »

See 1 Publication for this product

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