Anti-ATP6V1E1抗体(ab111733)
Key features and details
- Rabbit polyclonal to ATP6V1E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ATP6V1E1抗体
参阅全部 ATP6V1E1 一抗 -
描述
兔多克隆抗体to ATP6V1E1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant fragment within Human ATP6V1E1 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P36543 -
阳性对照
- A549 and Mouse brain whole cell lysates; HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111733于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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ICC/IF |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/500. |
靶标
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相关性
Function: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Tissue specificity: Ubiquitous. Similarity: Belongs to the V-ATPase E subunit family. -
细胞定位
Plasma membrane -
数据库链接
- Entrez Gene: 287017 Cow
- Entrez Gene: 529 Human
- Entrez Gene: 8992 Human
- Entrez Gene: 11973 Mouse
- Entrez Gene: 11974 Mouse
- Entrez Gene: 297566 Rat
- Entrez Gene: 94170 Rat
- Omim: 108746 Human
see all -
别名
- ATP6E antibody
- ATP6E2 antibody
- ATP6V1E antibody
see all
图片
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Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab111733 被引用在 6 文献中.
- Pottie L et al. Loss of zebrafish atp6v1e1b, encoding a subunit of vacuolar ATPase, recapitulates human ARCL type 2C syndrome and identifies multiple pathobiological signatures. PLoS Genet 17:e1009603 (2021). PubMed: 34143769
- Ponsford AH et al. Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. Autophagy N/A:1-19 (2020). PubMed: 32515674
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). PubMed: 29652249
- Doyle T et al. The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol 3:1369-1376 (2018). PubMed: 30478388
- Cao X et al. V-ATPase promotes transforming growth factor-ß-induced epithelial-mesenchymal transition of rat proximal tubular epithelial cells. Am J Physiol Renal Physiol 302:F1121-32 (2012). WB, ICC/IF ; Rat . PubMed: 22129967
- Sarkar J et al. V-type ATPase proton pump expression during enamel formation. Matrix Biol 52-54:234-245 (0). PubMed: 26586472