Anti-ATP6V0A4抗体(ab97440)
Key features and details
- Rabbit polyclonal to ATP6V0A4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-ATP6V0A4抗体
参阅全部 ATP6V0A4 一抗 -
描述
兔多克隆抗体to ATP6V0A4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 53 and 265 of Human ATP6V0A4 (NP_570856)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97440于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 96 kDa.
|
ICC/IF |
Use at an assay dependent concentration.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 96 kDa. |
ICC/IF
Use at an assay dependent concentration. |
靶标
-
相关性
ATP6V0A4 (ATPase, H+ transporting, lysosomal V0 subunit a isoform 4) is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. -
细胞定位
Apical cell membrane; Multi-pass membrane protein. -
数据库链接
- Entrez Gene: 50617 Human
- Entrez Gene: 140494 Mouse
- Omim: 605239 Human
- SwissProt: Q9HBG4 Human
- SwissProt: Q920R6 Mouse
-
别名
- A4 antibody
- ATP6N1B antibody
- ATP6N2 antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab97440 被引用在 3 文献中.
- Su K et al. Isoform a4 of the vacuolar ATPase a subunit promotes 4T1-12B breast cancer cell-dependent tumor growth and metastasis in vivo. J Biol Chem 298:102395 (2022). PubMed: 35988642
- McGuire CM et al. Isoform-specific gene disruptions reveal a role for the V-ATPase subunit a4 isoform in the invasiveness of 4T1-12B breast cancer cells. J Biol Chem 294:11248-11258 (2019). PubMed: 31167791
- Yanagisawa H et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci Rep 7:15944 (2017). PubMed: 29162837