概述

  • 产品名称
    Anti-ATP6IP2抗体
    参阅全部 ATP6IP2 一抗
  • 描述
    兔多克隆抗体to ATP6IP2
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-P, IHC-FoFr, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human ATP6IP2.

    (Peptide available as ab41522.)

  • 阳性对照
    • WB: Human brain, testis, skeletal muscle and liver tissue lysate. IHC-FoFr: Rat kidney tissue. IP: Rat kidney tissue lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 21628356ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. PubMed: 23077099
IHC-FoFr 1/100. See Abreview.
IHC-Fr Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能
    Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).
  • 组织特异性
    Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.
  • 疾病相关
    Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.
  • 翻译后修饰
    Phosphorylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APT6M8 9 antibody
    • APT6M8-9 antibody
    • ATP6AP2 antibody
    • ATP6IP2 antibody
    • ATP6M8-9 antibody
    • ATPase H(+)-transporting lysosomal accessory protein 2 antibody
    • ATPase H(+)-transporting lysosomal-interacting protein 2 antibody
    • ATPase H+ transporting lysosomal accessory protein 2 antibody
    • ATPase H+ transporting lysosomal interacting protein 2 antibody
    • ATPase H+ transporting lysosomal vacuolar proton pump membrane sector associated protein M8 9 antibody
    • ATPase membrane sector associated protein M8 9 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8 9 antibody
    • CAPER antibody
    • ELDF10 antibody
    • Embryonic liver differentiation factor 10 antibody
    • ER localized type I transmembrane adaptor antibody
    • ER-localized type I transmembrane adaptor antibody
    • HT028 antibody
    • M8 9 antibody
    • M8-9 antibody
    • MGC99577 antibody
    • MRXE antibody
    • MSTP009 antibody
    • N14F antibody
    • Renin receptor antibody
    • Renin/prorenin receptor antibody
    • RENR_HUMAN antibody
    • V ATPase M8 9 subunit antibody
    • V ATPase M8.9 subunit antibody
    • V-ATPase M8.9 subunit antibody
    • Vacuolar ATP synthase membrane sector associated protein M8 9 antibody
    • Vacuolar ATP synthase membrane sector-associated protein M8-9 antibody
    • vacuolar proton ATP synthase membrane sector associated protein M8 9 antibody
    • XMRE antibody
    see all

图片

  • ab40790 staining ATP6IP2 in rat kidney tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat. The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

  • All lanes : Anti-ATP6IP2 antibody (ab40790) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ab40790 staining ATP6IP2 in Human thoraci aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with Novacastra kit and blocked with Novacastra kit blocker for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody

    See Abreview

  • ATP6IP2 was immunoprecipitated using 0.5 mg rat kidney tissue lysate, 5 µg of Rabbit polyclonal to ATP6IP2 and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10 minutes, rat kidney tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70oC; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab40790.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 42 kDa; ATP6IP2

文献

This product has been referenced in:
  • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). IHC ; Human . Read more (PubMed: 28634582) »
  • Bradshaw AR  et al. Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin-Angiotensin System. Front Surg 3:51 (2016). Read more (PubMed: 27730123) »

See all 19 Publications for this product

客户评价及客户问答


The immunogen for ab40790 is C-terminal, thus it should be able to detect both the soluble and full length form of ATP6IP2. However, the 2 forms only have a difference of 1.5 kDa in MW, which is very hard to distinguish via WB. If a low primary an...

Read More

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and it is regrettable th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification
human thoraci aortic section- fresh frozen
Fixative
Acetone
Permeabilization
Yes - NOVACASTRA KIT COMPONENT
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Kidney sections)
Specification
Kidney sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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