概述

  • 产品名称
  • 描述
    兔多克隆抗体to ATP5H
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide between internal sequence amino acids 75-105 of Human ATP5H (NP_001003785.1 , NP_006347.1), conjugated to KLH.

  • 阳性对照
    • HepG2 cell line lysates; Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab107179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • 序列相似性
    Belongs to the ATPase d subunit family.
  • 细胞定位
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

图片

  • Anti-ATP5H antibody (ab107179) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab107179, at a 1/50 dilution, staining ATP5H in formalin fixed, paraffin embedded Human brain tissue by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.

文献

ab107179 has not yet been referenced specifically in any publications.

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