Anti-ATP5H抗体[7F9BG1] (ab110275)
Key features and details
- Mouse monoclonal [7F9BG1] to ATP5H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-ATP5H抗体[7F9BG1]
参阅全部 ATP5H 一抗 -
描述
小鼠单克隆抗体[7F9BG1] to ATP5H -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human, African green monkey -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
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常规说明
For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Near homogeneity as judged by SDS-PAGE. ab110275 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
7F9BG1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110275于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
|
ICC/IF |
Use a concentration of 1 - 5 µg/ml.
(heat-induced antigen-retrieval improvessignal) |
|
Flow Cyt | (1) |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. (heat-induced antigen-retrieval improvessignal) |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
序列相似性
Belongs to the ATPase d subunit family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282710 Cow
- Entrez Gene: 10476 Human
- Entrez Gene: 71679 Mouse
- Entrez Gene: 641434 Rat
- SwissProt: P13620 Cow
- SwissProt: O75947 Human
- SwissProt: Q9DCX2 Mouse
- SwissProt: P31399 Rat
see all -
别名
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
see all
图片
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All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size: 18 kDa -
All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution
Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 18 kDa -
Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
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HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab110275 被引用在 16 文献中.
- Lewis CJ et al. Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes. Redox Biol 30:101429 (2020). WB ; Human . PubMed: 31981894
- Ng R et al. Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues. Am J Physiol Heart Circ Physiol 318:H1516-H1524 (2020). PubMed: 32383992
- Chaphalkar RM et al. Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells. Sci Rep 10:3571 (2020). PubMed: 32107448
- Vilella R et al. Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes. Nutrients 12:N/A (2020). PubMed: 32993022
- Pecina P et al. Role of the mitochondrial ATP synthase central stalk subunits ? and d in the activity and assembly of the mammalian enzyme. Biochim Biophys Acta 1859:374-381 (2018). PubMed: 29499186