Anti-ATP5E抗体(ab54879)

概述

  • 产品名称Anti-ATP5E抗体
  • 描述
    小鼠单克隆抗体to ATP5E
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein: MVAYWRQAGL SYIRYSQICA KAVRDALKTE FKANAEKTSG SNVKIVKVKK E, corresponding to amino acids 1-52 of Human ATP5E

性能

应用

Our Abpromise guarantee covers the use of ab54879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the eukaryotic ATPase epsilon family.
    • 细胞定位Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP 5E antibody
      • ATP synthase epsilon chain mitochondrial antibody
      • ATP synthase H+ transporting mitochondrial F1 complex epsilon subunit antibody
      • ATP synthase subunit epsilon antibody
      • ATP5E antibody
      • ATP5E_HUMAN antibody
      • ATPase subunit epsilon antibody
      • ATPE antibody
      • F(0)F(1) ATPase antibody
      • H(+) transporting two sector ATPase antibody
      • MGC104243 antibody
      • mitochondrial antibody
      • Mitochondrial ATP synthase epsilon chain antibody
      • Mitochondrial ATPase antibody
      see all

    Anti-ATP5E antibody 图像

    • ATP5E antibody (ab54879) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.


    • Predicted band size : 6 kDa
      ATP5E antibody (ab54879) at 1ug/lane + SW-13 cell lysate at 25ug/lane.

    Anti-ATP5E antibody (ab54879)参考文献

    ab54879 has not yet been referenced specifically in any publications.

    Product Wall

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    You are right, it looks like the two products are made from the same clone. However, they differ in the concentration and purification method.

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    Application Western blot
    Sample Pig Tissue lysate - whole (myocardium)
    Loading amount 50 µg
    Specification myocardium
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Rahul Dalal

    Verified customer

    提交于 Sep 12 2012

    Thank you for contacting us.

    I am sorry that these two antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to con...

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    I am very pleased to hear you would like to accept our offer and test ab54879 inpig. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for pigand include this code in the &ld...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"