概述

  • 产品名称
  • 描述
    兔多克隆抗体to ATP5D
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment, corresponding to a region within amino acids 1-158 of Human ATP5D (NP_001678).

  • 阳性对照
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab97491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.

靶标

  • 功能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 序列相似性
    Belongs to the ATPase epsilon chain family.
  • 细胞定位
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
    • ATP5D antibody
    • ATPD_HUMAN antibody
    • F ATPase delta subunit antibody
    • F-ATPase delta subunit antibody
    • Mitochondrial ATP synthase complex delta subunit precusor antibody
    • Mitochondrial ATP synthase delta subunit antibody
    see all

图片

  • All lanes : Anti-ATP5D antibody (ab97491) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 17 kDa

实验方案

文献

ab97491 has not yet been referenced specifically in any publications.

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