Anti-ATP5D抗体(ab97491)
Key features and details
- Rabbit polyclonal to ATP5D
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ATP5D抗体
参阅全部 ATP5D 一抗 -
描述
兔多克隆抗体to ATP5D -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant protein fragment, corresponding to a region within amino acids 1-158 of Human ATP5D (NP_001678).
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阳性对照
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/250.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
IHC-P
1/250. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
序列相似性
Belongs to the ATPase epsilon chain family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 338081 Cow
- Entrez Gene: 513 Human
- Entrez Gene: 66043 Mouse
- Entrez Gene: 245965 Rat
- Omim: 603150 Human
- SwissProt: P05630 Cow
- SwissProt: P30049 Human
- SwissProt: Q9D3D9 Mouse
see all -
别名
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human breast cancer tissue, stained with ab97491 at 1/250.
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All lanes : Anti-ATP5D antibody (ab97491) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
12% SDS PAGE -
Anti-ATP5D antibody (ab97491) at 1/1000 dilution + Mouse brain at 50 µg
Predicted band size: 17 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab97491 被引用在 6 文献中.
- Ye Y et al. QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice. Front Pharmacol 12:759027 (2021). PubMed: 35095486
- Anand R et al. MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. Life Sci Alliance 3:N/A (2020). PubMed: 32788226
- Senapati P et al. Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer. Epigenetics Chromatin 12:44 (2019). PubMed: 31315653
- Tan JL et al. New High-Throughput Screening Identifies Compounds That Reduce Viability Specifically in Liver Cancer Cells That Express High Levels of SALL4 by Inhibiting Oxidative Phosphorylation. Gastroenterology 157:1615-1629.e17 (2019). PubMed: 31446059
- Liu X et al. Regulation of mitochondrial biogenesis in erythropoiesis by mTORC1-mediated protein translation. Nat Cell Biol 19:626-638 (2017). WB . PubMed: 28504707
- Engelen-Lee J et al. Proteomic profiling of the spinal cord in ALS: decreased ATP5D levels suggest synaptic dysfunction in ALS pathogenesis. Amyotroph Lateral Scler Frontotemporal Degener 18:210-220 (2017). PubMed: 27899032