Anti-ATP5A抗体[7H10BD4F9] (ab110273)

概述

  • 产品名称
    Anti-ATP5A抗体[7H10BD4F9]
    参阅全部 ATP5A 一抗
  • 描述
    小鼠单克隆抗体[7H10BD4F9] to ATP5A
  • 经测试应用
    适用于: IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Zebrafish
  • 免疫原

    Purified bovine ATP synthase

  • 阳性对照
    • Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 23515448
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • 组织特异性
    Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • 序列相似性
    Belongs to the ATPase alpha/beta chains family.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP synthase alpha chain, mitochondrial antibody
    • ATP synthase subunit alpha antibody
    • ATP synthase subunit alpha mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
    • ATP sythase (F1 ATPase) alpha subunit antibody
    • ATP5A antibody
    • Atp5a1 antibody
    • ATP5AL2 antibody
    • ATPA_HUMAN antibody
    • ATPM antibody
    • Epididymis secretory sperm binding protein Li 123m antibody
    • hATP1 antibody
    • HEL-S-123m antibody
    • MC5DN4 antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthetase antibody
    • Mitochondrial ATP synthetase oligomycin resistant antibody
    • Modifier of Min 2 mouse homolog antibody
    • Modifier of Min 2, mouse, homolog of antibody
    • MOM2 antibody
    • OMR antibody
    • ORM antibody
    • OTTHUMP00000163475 antibody
    see all

图片

  • All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg


    Predicted band size : 60 kDa
  • HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).

文献

This product has been referenced in:
  • Newman LE  et al. The abundance of the ARL2 GTPase and its GAP, ELMOD2, at mitochondria are modulated by the fusogenic activity of mitofusins and stressors. PLoS One 12:e0175164 (2017). Read more (PubMed: 28380071) »
  • Nguyen QL  et al. Platelets from pulmonary hypertension patients show increased mitochondrial reserve capacity. JCI Insight 2:e91415 (2017). Read more (PubMed: 28289721) »

See all 39 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 08 2017

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain, retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Brain, retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 08 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver, retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Brain, liver, retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 08 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
HEK cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 08 2017


Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is has not been determinated. The immunogen is a mixture of isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Hum...

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We do not have an antibody that has been tested for reactivity with E. coli ATP5A. The antibody ab14748, in addition to ab110273, gave good western blot results with other species. They may recognize E. coli ATP synthase alpha, but we do not guarantee ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cancer cells)
Specification
cancer cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2012


I am very pleased to hear you would like to accept our offer and test ab110273 in IP. This code will give you $XXX off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the ...

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Thank you for contacting Abcam (Mitosciences is now an Abcam company). I have contacted the lab in response to your email, asking about MS502(ab110237) and MS507 (ab14748). Unfortunately the epitopes that that these antibodies bind to have not ...

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Thank you for your enquiry. At this time we are not familiar with any use of ab110273 in IP. It certainly may work in this application. It is a good sign that the antibody is useful in Flow and ICC, as these application requires the antibody to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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