Anti-ATP5A抗体[15H4C4] - Mitochondrial Marker (FITC) (ab119688)

概述

  • 产品名称Anti-ATP5A抗体[15H4C4] - Mitochondrial Marker (FITC)
    参阅全部 ATP5A 一抗
  • 描述
    小鼠单克隆抗体[15H4C4] to ATP5A - Mitochondrial Marker (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Caenorhabditis elegans, Drosophila melanogaster, Monkey, Other
  • 免疫原

    Full length native protein (purified) corresponding to Cow ATP5A. Bovine Complex V.

  • 阳性对照
    • Flow Cyt: HeLa cells. ICC/IF: HeLa cells
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

    Alternative versions available:

    Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748)

性能

应用

Our Abpromise guarantee covers the use of ab119688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
Flow Cyt 1/100.

ab18427 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

靶标

  • 功能Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • 组织特异性Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • 序列相似性Belongs to the ATPase alpha/beta chains family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP synthase alpha chain, mitochondrial antibody
    • ATP synthase subunit alpha antibody
    • ATP synthase subunit alpha mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
    • ATP sythase (F1 ATPase) alpha subunit antibody
    • ATP5A antibody
    • Atp5a1 antibody
    • ATP5AL2 antibody
    • ATPA_HUMAN antibody
    • ATPM antibody
    • Epididymis secretory sperm binding protein Li 123m antibody
    • hATP1 antibody
    • HEL-S-123m antibody
    • MC5DN4 antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthetase antibody
    • Mitochondrial ATP synthetase oligomycin resistant antibody
    • Modifier of Min 2 mouse homolog antibody
    • Modifier of Min 2, mouse, homolog of antibody
    • MOM2 antibody
    • OMR antibody
    • ORM antibody
    • OTTHUMP00000163475 antibody
    see all

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (FITC) 图像

  • Overlay histogram showing HeLa cells stained with ab119688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab119688, 1/100 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG2b FITC (ab18419) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
  • ab119688 staining ATP5A in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab119688 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Mitochondrial localization of ATP5A using antibody ab119688. Cultured HeLa cells were fixed, permeabilized and then labeled with 15H4C4-FITC (1 µg/ml). Since the antibody is labeled with FITC no secondary antibody is necessary.
  • Overlay histogram showing HeLa cells stained with ab119688 (blue line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody ab119688 (15H4C4 FITC) at 1µg/1xE6/mL cells for 30 min at 22ºC. Negative control (red line) is unstained d cells. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (FITC) (ab119688)参考文献

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