The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Belongs to the X(+)/potassium ATPases subunit beta family.
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.