Synthetic peptide between N terminal amino acids 108-137 of Human ATP13A3 (NP_078800.3), conjugated to KLH.
HepG2 cell line lysates; Human bladder carcinoma
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500. Predicted molecular weight: 138 kDa.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.
Information by UniProt
ATPase family homolog up-regulated in senescence cells 1 antibody
Probable cation-transporting ATPase 13A3 antibody
Western blot - ATP13A3 antibody (ab107178)
Anti-ATP13A3 antibody (ab107178) at 1/100 dilution + HepG2 cell line lysates at 35 µg
Predicted band size: 138 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP13A3 antibody (ab107178)
ab107178, at a 1/50 dilution, staining ATP13A3 in formalin fixed, paraffin embedded Human bladder carcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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