概述

  • 产品名称Anti-ATP10D抗体
    参阅全部 ATP10D 一抗
  • 描述
    兔多克隆抗体to ATP10D
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 1151-1200 (NLLFTSAPPV IYGVLEKDVS AETLMQLPEL YRSGQKSEAY LPHTFWITLL) of Human ATP10D (NP_065186).

  • 阳性对照
    • Fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab81174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65, >168 kDa (predicted molecular weight: 160 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

靶标

  • 相关性The exact function of ATP10D remains unknown.
  • 细胞定位Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not that of TMEM30B.
  • 数据库链接
  • 别名
    • ATP10D antibody
    • ATPase class V type 10D antibody
    • ATPVD antibody
    • KIAA1487 antibody
    • Probable phospholipid transporting ATPase VD antibody
    • Putative type IV aminophospholipid transporting ATPase antibody
    • Type IV aminophospholipid transporting ATPase antibody
    see all

Anti-ATP10D antibody 图像

  • Anti-ATP10D antibody (ab81174) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 160 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

Anti-ATP10D antibody (ab81174)参考文献

ab81174 has not yet been referenced specifically in any publications.

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