概述

  • 产品名称ATP Synthase免疫捕获试剂盒
    参阅全部 ATP Synthase 试剂盒
  • 检测类型Quantitative
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.

    ab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • 经测试应用适用于: IPmore details

性能

应用

Our Abpromise guarantee covers the use of ab109715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.

ATP Synthase Immunocapture Kit 图像

  • Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.

实验方案

ATP Synthase Immunocapture Kit (ab109715)参考文献

This product has been referenced in:
  • Sheeran FL & Pepe S Posttranslational modifications and dysfunction of mitochondrial enzymes in human heart failure. Am J Physiol Endocrinol Metab 311:E449-60 (2016). Read more (PubMed: 27406740) »
  • Alavian KN  et al. An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore. Proc Natl Acad Sci U S A 111:10580-5 (2014). Read more (PubMed: 24979777) »

See all 7 Publications for this product

Product Wall


1. We do not know the detail epitope of this antibody, it pulls down the full complex of the ATP synthase.
2. It is possible to target on the subunits of F1 fraction but very unlikely to IP the whole F1 fraction only with this product. Instead...

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You are correct that you cannot use B-mercaptoethanol since it also reduces the disulfide bonds of IgG. The binding between antibodies and ATP synthase can be dissociate with SDS or Glycine buffer. These buffers will not affect disulfide bond but o...

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The lab has confirmed that some IgG will come off (this is inevitable to some degree even though they are chemically cross linked to the beads), this can be minimized by washing the beads thoroughly after capture and by not using DTT in the SDS elution...

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Thank you for your enquiry

Lauryl Maltoside is a non-ionic detergent which solubilize proteins but is able to keep the protein complex intact. MS501 immunocaptures ATP synthase as a complex, therefore, Lauryl Maltoside is a critical detergent...

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Product code: 109714 Inquiry: Hi Abcam, Do you know if this kit will immunocapture the ATP synthase enzyme from C. elegans? Unlikely unfortunately – this kit was tested positive with human, rat and cow. Mouse was negative. I think it is unl...

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Thank you for contacting us.

We do not currently offer the protein G- agarose beads used in this kit. Any commercially available protein-G agarose beads should work as control. An excellent resource for finding those is Biocompare.com.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"