ATP synthase Enzyme Activity Microplate Assay试剂盒(ab109714)


  • 产品名称ATP synthase Enzyme Activity Microplate Assay试剂盒
    参阅全部 ATP Synthase 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Cell Lysate, Purified mitochondria
  • 检测类型Enzyme activity
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat, Cow, Human
    不与反应: Mouse
  • 产品概述

    ab109714 ATP synthase Enzyme Activity Microplate Assay Kit is used to determine the activity of ATP synthase (Complex V) in a human or rat sample. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.


  • 说明

    Store Buffer (Tube 1), Detergent, and the covered microplate at 4°C. Store the Lipid Mix and Reagent Mix at -80°C. For multiple experiments the Reagent Mix may be aliquoted at -80°C.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate



Our Abpromise guarantee covers the use of ab109714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

ATP synthase Enzyme Activity Microplate Assay Kit 图像

  • Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
  • Functional study using ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).
  • Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).


ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)参考文献

This product has been referenced in:
  • Yao X  et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS One 10:e0139416 (2015). Functional Studies ; Rat . Read more (PubMed: 26448624) »
  • Zhao Y  et al. Redox proteomic identification of HNE-bound mitochondrial proteins in cardiac tissues reveals a systemic effect on energy metabolism after doxorubicin treatment. Free Radic Biol Med 72:55-65 (2014). Functional Studies . Read more (PubMed: 24632380) »

See all 9 Publications for this product

Product Wall

Thank you for your telephone enquiry earlier today.

I contacted the laboratory who confirmed that the 10X detergent included with this kit is 5% Lauryl Maltoside. The closest product we have is 10% Lauryl Maltoside Solution (ab109857). http:/...

Read More

Yes, typically researchers have used citrate synthase activity to normalize the quality of sample preparation. However, it is also well known in the literature that cell lines with defects in the OXPHOS complexes have increase citrate synthase activity...

Read More

- No hay ningún problema en realizar el ensayo a 37 ºC. Simplemente irá mas rápido y no podréis comparar los resultados obtenidos con los de la datasheet, o con publicaciones anteriores que pudieran resultaros...

Read More

En respuesta a las preguntas formuladas acerca del kit ab109714:

- Una de ellas hace referencia a la temperatura, ¿por qué el ensayo es a 30ºC? ¿Las enzimas que se utilizan no son de mamífero? El ens...

Read More

Any color development is not visible to the naked eye but should easily be detectable to the plate reader.

The wavelength looks right 340nm and there should be a sizeable absorbance due to NADH abs at 340nm (we would expect 0.5), lo...

Read More

Thank you for contacting us.Please find the replies to your questions below.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1. H...

Read More

Product code: 109714 Inquiry: Hi Abcam, Do you know if this kit will immunocapture the ATP synthase enzyme from C. elegans? Unlikely unfortunately – this kit was tested positive with human, rat and cow. Mouse was negative. I think it is unl...

Read More

Thank you for your response.

You can certainly freeze down the samples if you wish. I have attached a new version of the Protocol which will be updated/downloaded on the on-line product datasheet as well.

If you need any further ass...

Read More

Thank you for your enquiry and your interest in our products.

I would suggest trypsinizing the cells to detach them from the culture dish/well or using a cell scraper.

Afterwards the cells can be pelleted and washed, then brought up...

Read More

The ATP synthase kit ab109714 is not suitable for mouse samples, as the capture antibody in the kit will not recognize mouse synthase.

Please let me know if you have any other questions.