RabMAb

Anti-ATP citrate lyase抗体[EP704Y] (ab40793)

概述

  • 产品名称Anti-ATP citrate lyase抗体[EP704Y]
    参阅全部 ATP citrate lyase 一抗
  • 描述
    兔单克隆抗体[EP704Y] to ATP citrate lyase
  • 特异性ab40793 recognises ATP citrate lyase (ACL).
  • 经测试应用适用于: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Marmoset (common)
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP citrate lyase aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab207504)

  • 阳性对照
    • WB: HeLa cell lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: Jurkat cell lysate.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab40793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
IHC-P 1/100. PubMed: 19727777
WB 1/1000 - 1/5000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

靶标

  • 功能ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • 序列相似性In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • 翻译后修饰ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Anti-ATP citrate lyase antibody [EP704Y] 图像

  • Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution + HeLa cell lysate

    Predicted band size : 122 kDa
    Observed band size : 122 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATP citrate lyase with ab40793 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Overlay histogram showing HeLa cells stained with ab40793 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40793, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab40793 at 1/40 immunoprecipitating ATP citrate lyase in HeLa (human cervix adenocarcinoma) whole cell lysate.

    Lane 1 (input): HeLa (human cervix adenocarcinoma) whole cell lysate 10μg

    Lane 2 (+): ab40793 + HeLa (human cervix adenocarcinoma) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa (human cervix adenocarcinoma) whole cell lysate

    For western blotting, ab40793 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/10000.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-ATP citrate lyase antibody [EP704Y] (ab40793)参考文献

This product has been referenced in:
  • Walsh J  et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . Read more (PubMed: 24859727) »
  • Daniëls VW  et al. Cancer cells differentially activate and thrive on de novo lipid synthesis pathways in a low-lipid environment. PLoS One 9:e106913 (2014). WB ; Human . Read more (PubMed: 25215509) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Marmoset (common) Tissue lysate - whole (brain)
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2014

Vielen Dank für Ihren Anruf.

Leider ermitteln wir für diesen Antikörper die Konzentration nicht, so dass ich Ihnen leider in diesem Fall nicht weiterhelfen kann.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie...

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Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount 40 µg
Specification Head
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 02 2012


Please log into your Abcam account, and than open the datasheet for the antibody you would like to write a review about. On top of the datasheet, you will find five tags : Datasheet, specific references, Abreview, Scientific Support, Protocols. Read More

Expiration date: 08. September 2012

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