Anti-ATM (phospho S794)抗体(ab119799)


  • 产品名称Anti-ATM (phospho S794)抗体
    参阅全部 ATM 一抗
  • 描述
    兔多克隆抗体to ATM (phospho S794)
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide conjugated to carrier protein, corresponding to amino acids surrounding S794 in Human ATM.

  • 阳性对照
    • Jurkat, A431, HeLa, and Rat PC12 cells, treated with Calyculin A.
  • 常规说明Do not aliquot.



Our Abpromise guarantee covers the use of ab119799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 351 kDa.
ICC 1/200.


  • 功能Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
  • 组织特异性Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
  • 疾病相关Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
    Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
    Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
    Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
  • 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 PI3K/PI4K domain.
  • 结构域The FATC domain is required for interaction with KAT5.
  • 翻译后修饰Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
    Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
  • 细胞定位Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A-T mutated antibody
    • A-T mutated homolog antibody
    • AT mutated antibody
    • AT1 antibody
    • ATA antibody
    • Ataxia telangiectasia mutated antibody
    • Ataxia telangiectasia mutated gene antibody
    • Ataxia telangiectasia mutated homolog (human) antibody
    • Ataxia telangiectasia mutated homolog antibody
    • ATC antibody
    • ATD antibody
    • ATDC antibody
    • ATE antibody
    • ATM antibody
    • ATM serine/threonine kinase antibody
    • ATM_HUMAN antibody
    • DKFZp781A0353 antibody
    • MGC74674 antibody
    • OTTHUMP00000232981 antibody
    • Serine protein kinase ATM antibody
    • Serine-protein kinase ATM antibody
    • Serine/threonine-protein kinase ATM antibody
    • Tefu antibody
    • TEL1 antibody
    • TEL1, telomere maintenance 1, homolog antibody
    • TELO1 antibody
    • Telomere fusion protein antibody
    see all

Anti-ATM (phospho S794) antibody 图像

  • ab119799 at 1/200 dilution staining ATM (phospho S794) in A431 cells treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to DyLight 594.
  • Lanes 1 - 2 : Anti-ATM (phospho S794) antibody (ab119799) at 1/1000 dilution
    Lanes 3 - 4 : Anti- ATM (C-Terminal) at 1/1000 dilution
    Lanes 5 - 6 : Anti-ATM (phospho S1981) at 1/1000 dilution

    Lane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min, then treated with lambda phosphatase
    Lane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min, then treated with lambda phosphatase
    Lane 5 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 6 : Human A431 cells treated with Calyculin A (100 nM) for 30 min, then treated with lambda phosphatase

    Predicted band size : 351 kDa

Anti-ATM (phospho S794) antibody (ab119799)参考文献

ab119799 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. This refers to the image on our datasheet:

Unfortunately, the lab was unable to provide a copy that had not...

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Thank you foryour patience.

For ab119799, the band on our datasheet is running at 370 kDa, verified by comparison to Bio-Rad rainbow markers and co-migration with the other ATM antibodies shown. In human A431 cells, we do observe a lower doub...

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Thank you for your reply.

These cells were not exposed to any other compounds besides calyculin A. The notes included in our previous correspondance refers more generally to ATM activation.

I hope this information is hel...

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Thank you for contacting Abcam earlier today.

The image of ab119799 shows bands running near 370 kDa, these bands are a good bit higher than our 220 kDa molecular weight standard which is not shown. We treat with 100 nM Calyculin A (serine an...

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