Anti-ATM抗体[Y170] (ab32420)


  • 产品名称Anti-ATM抗体[Y170]
    参阅全部 ATM 一抗
  • 描述
    兔单克隆抗体[Y170] to ATM
  • 特异性This antibody is specific for ATM.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ATM.
    (Peptide available as ab170988)

  • 阳性对照
    • WB: 293 cell lysate. FC: HeLa cells
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 350 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.


Flow Cyt 1/20.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
    • 组织特异性Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
    • 疾病相关Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
      Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
      Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
      Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
    • 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 PI3K/PI4K domain.
    • 结构域The FATC domain is required for interaction with KAT5.
    • 翻译后修饰Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
      Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
    • 细胞定位Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A-T mutated antibody
      • A-T mutated homolog antibody
      • AT mutated antibody
      • AT1 antibody
      • ATA antibody
      • Ataxia telangiectasia mutated antibody
      • Ataxia telangiectasia mutated gene antibody
      • Ataxia telangiectasia mutated homolog (human) antibody
      • Ataxia telangiectasia mutated homolog antibody
      • ATC antibody
      • ATD antibody
      • ATDC antibody
      • ATE antibody
      • ATM antibody
      • ATM serine/threonine kinase antibody
      • ATM_HUMAN antibody
      • DKFZp781A0353 antibody
      • MGC74674 antibody
      • OTTHUMP00000232981 antibody
      • Serine protein kinase ATM antibody
      • Serine-protein kinase ATM antibody
      • Serine/threonine-protein kinase ATM antibody
      • Tefu antibody
      • TEL1 antibody
      • TEL1, telomere maintenance 1, homolog antibody
      • TELO1 antibody
      • Telomere fusion protein antibody
      see all

    Anti-ATM antibody [Y170] 图像

    • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ATM with ab32420 at 1:100 dilution. Tissue underwent antigen retrieval using Tris/EDTA Buffer (pH9.0). The section was counterstained with haematoxylin.

    • Immunofluorescent staining of HeLa cells (fixed with 4% PFA) with purified ab32420 at a dilution of 1/250. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    • All lanes : Anti-ATM antibody [Y170] (ab32420) at 1/3000 dilution (Purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HEK293 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 350 kDa
      Observed band size : 370 kDa (why is the actual band size different from the predicted?)
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling ATM with purified ab32420 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling ATM with ab32420 at 1:100 dilution. Tissue underwent antigen retrieval using Tris/EDTA Buffer (pH9.0). The section was counterstained with haematoxylin.

    Anti-ATM antibody [Y170] (ab32420)参考文献

    This product has been referenced in:
    • Feng X  et al. Low Ki67/high ATM protein expression in malignant tumors predicts favorable prognosis in a retrospective study of early stage hormone receptor positive breast cancer. Oncotarget 7:85798-85812 (2016). IHC ; Human . Read more (PubMed: 27741524) »
    • Zhang H  et al. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome. PLoS One 11:e0167454 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27907109) »

    See all 27 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA Buffer
    Sample Human Tissue sections (Serous Ovarian Tumour)
    Specification Serous Ovarian Tumour
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Jul 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line
    Gel Running Conditions Reduced Denaturing (7%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Christian Marx

    Verified customer

    提交于 Oct 29 2012

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization No
    Specification Colon
    Blocking step hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative Formaldehyde

    Vinicius Kannen Cardoso

    Verified customer

    提交于 Aug 10 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9 20 mins Leica EDTA
    Permeabilization No
    Specification Colon
    Blocking step (agent) for 5 minute(s) · Concentration: 10%
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Feb 29 2016

    The blocking peptide for the Anti-ATM antibody [Y170] (ab32420) has now been added to the catalogue following your enquiry. This can be accessed under the catalogue number ab170988

    For producing the IHC-P images, including the glioma tissue, on the datasheet for ab32420, we used the following protocol with a sodium citrate heat mediated antigen retrieval:
    We had success w...

    Read More

    Gracias por tu respuesta.
    Efectivamente, si haces un pedido de ab26, el ab32420 os saldría gratuito, al entrar en la promoción de RabMabs.
    Simplemente indica el código promocional RABMAB-XBSMG en la casilla destinada para ello cuando realizas...

    Read More

    Thank you for contacting us.
    Unfortunately I cannot find this particular lot number for this product. I have checked the orders from your Organisation for this antibody and found an order from December 2011. The lot number for the vial sent is XXX...

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    Thank you for contacting us and your interest in our products.

    Epitomics did offer isotype controls specific for different lots of antibody purchased. This was providing the antibody at the same concentration as the antibody to be tested. We d...

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    Thank you for contacting Abcam.

    The peptides for both ab32420 and ab81292 maps between amino acids ***********of human ATM (UniProt: Q13315).

    Please let me know if there is anything else I can help you with.