Anti-ATM抗体(ab2631)

概述

• 产品名称Anti-ATM抗体
  参阅全部 ATM 一抗
• 描述
  山羊多克隆抗体to ATM
• 经测试应用适用于: WB, IPmore details
• 种属反应性
  与反应: Mouse, Human
  预测可用于: Chimpanzee
• 免疫原

  Synthetic peptide (Human) - which represents a portion of human Ataxia Telangiectasia Mutated (ATM) proteinencoded within exon 53.

• 阳性对照
  • Positive controls: Human L-40 or mouse MF-10 wildtype whole cell lysates. Negative controls: Human L-6 or mouse MF-12 ATM deficient whole cell lysates.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液Preservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• 纯化说明Affinity purified using the immunising peptide immobilized on solid support.
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • ATM / ATR

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

相关产品

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

应用

靶标

• 功能Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
• 组织特异性Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
• 疾病相关Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
  Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
  Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
  Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
• 序列相似性Belongs to the PI3/PI4-kinase family. ATM subfamily.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 1 PI3K/PI4K domain.
• 结构域The FATC domain is required for interaction with KAT5.
• 翻译后修饰Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
  Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
• 细胞定位Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

• Target information above from: UniProt accession Q13315 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 472 Human
  • Entrez Gene: 11920 Mouse
  • Omim: 607585 Human
  • SwissProt: Q13315 Human
  • SwissProt: Q62388 Mouse
  • Unigene: 367437 Human
  • Unigene: 5088 Mouse
• 别名
  • A-T mutated antibody
  • A-T mutated homolog antibody
  • AT mutated antibody

  • AT1 antibody
  • ATA antibody
  • Ataxia telangiectasia mutated antibody
  • Ataxia telangiectasia mutated gene antibody
  • Ataxia telangiectasia mutated homolog (human) antibody
  • Ataxia telangiectasia mutated homolog antibody
  • ATC antibody
  • ATD antibody
  • ATDC antibody
  • ATE antibody
  • ATM antibody
  • ATM serine/threonine kinase antibody
  • ATM_HUMAN antibody
  • DKFZp781A0353 antibody
  • MGC74674 antibody
  • OTTHUMP00000232981 antibody
  • Serine protein kinase ATM antibody
  • Serine-protein kinase ATM antibody
  • Serine/threonine-protein kinase ATM antibody
  • Tefu antibody
  • TEL1 antibody
  • TEL1, telomere maintenance 1, homolog antibody
  • TELO1 antibody
  • Telomere fusion protein antibody

  see all