Synthetic peptide, corresponding to a region within amino acids 491-541 of Human ATL3 (NP_056274.3).
HeLa, Jurkat, 293T and Mouse NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117834 was affinity purified using an epitope specific to ATL3 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
Use at 2-10 µg/mg of lysate.
GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
Expressed in peripheral tissues (at protein level).
Belongs to the GBP family. Atlastin subfamily.
Endoplasmic reticulum membrane.
Information by UniProt
Western blot - Anti-ATL3 antibody (ab117834)
All lanes : Anti-ATL3 antibody (ab117834) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 61 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-ATL3 antibody (ab117834)
Detection of Human ATL3 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab117834 at 6 µg/mg of lysate. Subsequent WB detection was done using 117834 at 1 µg/ml. ECL detection. Exposure time: 10 seconds.
has not yet been referenced specifically in any publications.
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