概述

  • 产品名称Anti-ATG4B抗体
    参阅全部 ATG4B 一抗
  • 描述
    兔多克隆抗体to ATG4B
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 350-393 of Human ATG4B. (NP_037457).

  • 阳性对照
    • HeLa and RAW cell lysates and human heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab72201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • 组织特异性Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • 序列相似性Belongs to the peptidase C54 family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Anti-ATG4B antibody 图像

  • ab72201 at 10µg/ml staining ATG4B in human heart by Immunohistochemistry, Paraffin-embedded tissue.
  • All lanes : Anti-ATG4B antibody (ab72201) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with immunizing peptide
    Lane 3 : RAW cell lysate


    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

Anti-ATG4B antibody (ab72201)参考文献

ab72201 has not yet been referenced specifically in any publications.

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