概述

  • 产品名称Anti-ATG4B抗体
    参阅全部 ATG4B 一抗
  • 描述
    兔多克隆抗体to ATG4B
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the central region of human ATG4B conjugated to KLH

  • 阳性对照
    • 293 cell lysates (nontransfected or transiently transfected with the ATG4B gene) and human skeletal muscle tissue.

性能

应用

Our Abpromise guarantee covers the use of ab70867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • 组织特异性Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • 序列相似性Belongs to the peptidase C54 family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Anti-ATG4B antibody 图像

  • All lanes : Anti-ATG4B antibody (ab70867) at 1/50 dilution

    Lane 1 : 293 cell lysates (nontransfected)
    Lane 2 : 293 cell lysates transiently transfected with the ATG4B gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • abx at 1/10 dilution, staining ATG4B in human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-ATG4B antibody (ab70867)参考文献

This product has been referenced in:
  • Rothe K  et al. The core autophagy protein ATG4B is a potential biomarker and therapeutic target in CML stem/progenitor cells. Blood 123:3622-34 (2014). WB ; Human . Read more (PubMed: 24755409) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70867.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"