概述

  • 产品名称Anti-ATG2B抗体
    参阅全部 ATG2B 一抗
  • 描述
    兔多克隆抗体to ATG2B
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 117-146 of Human ATG2B (NP_060506.5).

  • 阳性对照
    • CEM cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab116215 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 233 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 序列相似性Belongs to the ATG2 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410024A21Rik antibody
    • AI047755 antibody
    • AI503411 antibody
    • ATG2 autophagy related 2 homolog B antibody
    • Atg2b antibody
    • ATG2B_HUMAN antibody
    • Autophagy-related protein 2 homolog B antibody
    • AW558123 antibody
    • C030004M05Rik antibody
    • C14orf103 antibody
    • C630028L02Rik antibody
    • FLJ10242 antibody
    • KIAA4067 antibody
    • mKIAA4067 antibody
    see all

Anti-ATG2B antibody 图像

  • Anti-ATG2B antibody (ab116215) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 233 kDa
  • ab116215 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116215 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ATG2B antibody (ab116215)参考文献

ab116215 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116215.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"