概述

  • 产品名称Anti-ATG16L1抗体
    参阅全部 ATG16L1 一抗
  • 描述
    兔多克隆抗体to ATG16L1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ATG16L1.

    (Peptide available as ab48088.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human lung; Human placenta

性能

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应用

Our Abpromise guarantee covers the use of ab47946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • 疾病相关Inflammatory bowel disease 10
  • 序列相似性Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • 翻译后修饰Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • 细胞定位Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • 数据库链接
  • 形式There are 4 isoforms produced by alternative splicing.
  • 别名
    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Anti-ATG16L1 antibody 图像

  • ATG16L1 was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to ATG16L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47946.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 57kDa; ATG16L1

  • All lanes : Anti-ATG16L1 antibody (ab47946) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,40 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    This antibody was raised against an immunogen that is predicted to recognize isoforms (1-5) of Human ATG16L1. The predicted molecular weights of isoforms (1-5) are 68, 66, 53,47 kDa and 49 kDa respectively.

Anti-ATG16L1 antibody (ab47946)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embyonic fibroblast)
Gel Running Conditions Reduced Denaturing
Loading amount 1 µg
Specification mouse embyonic fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Dr. Ryan Russell

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提交于 Sep 02 2016

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regretta...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 cells)
Loading amount 10 µg
Specification A549 cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (P388D1 cells, macrophages)
Loading amount 10 µg
Specification P388D1 cells, macrophages
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 24 2010

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7 cell line)
Loading amount 20 µg
Specification COS-7 cell line
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 25 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Tissue lysate - whole (hamster muscle tissue)
Loading amount 20 µg
Specification hamster muscle tissue
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2009

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