Anti-ATG16L1抗体(ab188642)
Key features and details
- Rabbit polyclonal to ATG16L1
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ATG16L1抗体
参阅全部 ATG16L1 一抗 -
描述
兔多克隆抗体to ATG16L1 -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human ATG16L1 (internal sequence). 18 amino acids.
Database link: Q676U5 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188642于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 2 - 4 µg/ml.
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WB |
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 2 - 4 µg/ml. |
WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. -
疾病相关
Inflammatory bowel disease 10 -
序列相似性
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats. -
翻译后修饰
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. -
细胞定位
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. - Information by UniProt
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数据库链接
- Entrez Gene: 55054 Human
- Entrez Gene: 77040 Mouse
- Entrez Gene: 363278 Rat
- Omim: 610767 Human
- SwissProt: Q676U5 Human
- SwissProt: Q8C0J2 Mouse
- Unigene: 529322 Human
- Unigene: 272972 Mouse
see all -
形式
There are 4 isoforms produced by alternative splicing. -
别名
- A16L1_HUMAN antibody
- APG16 like 1 antibody
- APG16-like 1 antibody
see all
图片
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Lane 1 : Anti-ATG16L1 antibody (ab188642) at 0.25 µg/ml
Lane 2 : Anti-ATG16L1 antibody (ab188642) at 0.5 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 68 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
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Immunocytochemical/ Immunofluorescence analysis of HeLa cells labeling ATG16L1 using ab188642 at 4 µg/ml.
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Immunocytochemical analysis of HeLa cells labeling ATG16L1 using ab188642 at 2 µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab188642 被引用在 7 文献中.
- Zhao J et al. miR-130a-3p, a Preclinical Therapeutic Target for Crohn's Disease. J Crohns Colitis 15:647-664 (2021). PubMed: 33022049
- He Z et al. MicroRNA 223 Targeting ATG16L1 Affects Microglial Autophagy in the Kainic Acid Model of Temporal Lobe Epilepsy. Front Neurol 12:704550 (2021). PubMed: 34381417
- Ou M et al. Emerging roles of let-7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation. Int J Mol Med 46:83-96 (2020). PubMed: 32319531
- Liu K et al. MicroRNA-106a Inhibits Autophagy Process and Antimicrobial Responses by Targeting ULK1, ATG7, and ATG16L1 During Mycobacterial Infection. Front Immunol 11:610021 (2020). PubMed: 33505399
- Pellegrini D et al. Quantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease. Mol Cell Proteomics 18:1227-1241 (2019). PubMed: 30926673
- Zhang K et al. PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell Death Dis 9:312 (2018). PubMed: 29472524
- Liu H et al. Oxidative Stress Mediates Microcystin-LR-Induced Endoplasmic Reticulum Stress and Autophagy in KK-1 Cells and C57BL/6 Mice Ovaries. Front Physiol 9:1058 (2018). PubMed: 30131715