概述

  • 产品名称Anti-ATF7抗体
    参阅全部 ATF7 一抗
  • 描述
    兔多克隆抗体to ATF7
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 444-494 (ATAPSNGLSV RSAAEAVATS VLTQMASQRT ELSMPIQSHV IMTPQSQSAG R) of human ATF7 (CAA40483.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab87844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
      Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
    • 组织特异性Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
    • 序列相似性Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • 翻译后修饰On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
      Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
    • 细胞定位Nucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1110012F10Rik antibody
      • 9430065F09Rik antibody
      • Activating transcription factor 7 antibody
      • AI549878 antibody
      • ATF7 antibody
      • Atf7 protein antibody
      • ATF7_HUMAN antibody
      • ATFA antibody
      • C130020M04Rik antibody
      • cAMP-dependent transcription factor ATF-7 antibody
      • Cyclic AMP-dependent transcription factor ATF 7 antibody
      • Cyclic AMP-dependent transcription factor ATF-7 antibody
      • MGC31554 antibody
      • MGC57182 antibody
      • Transcription factor ATF A antibody
      • Transcription factor ATF-A antibody
      see all

    Anti-ATF7 antibody 图像

    • All lanes : Anti-ATF7 antibody (ab87844) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
      Developed using the ECL technique, with an exposure time of 10 seconds.

    Anti-ATF7 antibody (ab87844)参考文献

    ab87844 has not yet been referenced specifically in any publications.

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