概述

  • 产品名称Anti-ATF6抗体[1-7]
    参阅全部 ATF6 一抗
  • 描述
    小鼠单克隆抗体[1-7] to ATF6
  • 特异性specific to human ATF6 alfa no cross reactivity with mouse ATF6 alfa.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human ATF6 (N terminal). Epitope is not determined

  • 阳性对照
    • 293T, HeLa S3 Tet-off, 293 and HeLa cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯化说明ab122897 was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes, then filter sterilized.
  • 克隆单克隆
  • 克隆编号1-7
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.

If clear result not obtained Immunoprecipitation may help

IP Use at an assay dependent concentration.
ICC/IF 1/100.

靶标

  • 功能Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • 结构域The basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • 翻译后修饰During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
    N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
    Phosphorylated in vitro by MAPK14/P38MAPK.
  • 细胞定位Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating transcription factor 6 alpha antibody
    • Activating transcription factor 6 antibody
    • ATF 6 antibody
    • ATF6 alpha antibody
    • ATF6 antibody
    • ATF6-alpha antibody
    • ATF6A antibody
    • ATF6A_HUMAN antibody
    • cAMP dependent transcription factor ATF 6 alpha antibody
    • cAMP-dependent transcription factor ATF-6 alpha antibody
    • Cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • DKFZp686P2194 antibody
    • ESTM49 antibody
    • FLJ21663 antibody
    • Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
    see all

Anti-ATF6 antibody [1-7] 图像

  • Immunofluorescent analysis of HeLa cells using ab122897 at a dilution of 1/100. The cells were fixed with paraformaldehyde. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG at a dilution of 1/1000. The antibody staining is shown in the top panel, and the merged image with DAPI counter-staining is shown in the lower panel.

  • All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution

    Lane 1 : 293T cell lysate: control
    Lane 2 : 293T cell lysate: treated with 2 µg/ml tunicamycin for 7 hours
    Lane 3 : 293T cell lysate: treated with 1mM DTT for 1 hour.
    Lane 4 : HeLa S3 Tet-off cell lysate: control
    Lane 5 : HeLa S3 Tet-off cell lysate: treated with 1mM DTT for 1 hour.


    Predicted band size : 75 kDa
    Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells.
  • All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution

    Lane 1 : HeLa cell extract: control
    Lane 2 : HeLa cell extract: treated with 1mM DTT for 1 hour
    Lane 3 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 3 hour
    Lane 4 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 7 hour
    Lane 5 : 293 cell extract: control
    Lane 6 : 293 cell extract: treated with 1mM DTT for 1 hour
    Lane 7 : 293 cell extract: treated with 2 µg/ml tunicamycin for 3 hour
    Lane 8 : 293 cell extract: treated with 2 µg/ml tunicamycin for 7 hour


    Predicted band size : 75 kDa
    Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells.
  • ATF6 was detected by Western blot (Input; lanes 1, 2, 7, and 8) using ab122897 (No. 1-7). After immunoprecipitation (IP) with non-related IgG (IP; lanes 3, 4, 9, and 10) or ab122897 (No. 1-7) (IP; lanes 5, 6, 11, and 12), samples were subjected to SDS-PAGE and analyzed by Western blot using ab122897 (No. 1-7) and anti-mouse IgG antibody (light chain specific).

Anti-ATF6 antibody [1-7] (ab122897)参考文献

This product has been referenced in:
  • Karam R  et al. The unfolded protein response is shaped by the NMD pathway. EMBO Rep 16:599-609 (2015). WB . Read more (PubMed: 25807986) »
  • Gupta A  et al. PERK regulated miR-424(322)-503 cluster fine-tunes activation of IRE1 and ATF6 during Unfolded Protein Response. Sci Rep 5:18304 (2015). WB . Read more (PubMed: 26674075) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (DU145)
Permeabilization Yes - 0.2% Triton X-100
Specification DU145
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 31 2016

Application Western blot
Sample Human Cell lysate - whole cell (HBEC)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 50 µg
Treatment glucose deprivation
Specification HBEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 08 2016

Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing
Sample African Green Monkey Cell lysate - whole cell (Cox7)
Specification Cox7
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1.25% · Temperature: 25°C
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提交于 Nov 24 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Cell lysate - whole cell (lymphocytes)
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Jul 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"