Anti-ATF2 (phospho T71 + T53)抗体(ab28812)


  • 产品名称Anti-ATF2 (phospho T71 + T53)抗体
    参阅全部 ATF2 一抗
  • 描述
    兔多克隆抗体to ATF2 (phospho T71 + T53)
  • 经测试应用适用于: WB, IHC-P, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized phospho peptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.

  • 阳性对照
    • IHC-P: Human breast carcinoma. WB: HeLa cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium Azide.
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab28812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
IP Use at an assay dependent concentration.


  • 功能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 组织特异性Abundant expression seen in the brain.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻译后修饰Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 (phospho T71 + T53) antibody 图像

  • Ab28812, at a dilution of 1/50, staining ATF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Left image: using the ATF2 antibody
    Right image: using the same antibody preincubated with synthesized phospho peptide.
  • All lanes : Anti-ATF2 (phospho T71 + T53) antibody (ab28812) at 1/1000 dilution

    Lane 1 : untreated HeLa cells (5-30ug lysate).
    Lane 2 : HeLa cells treated with UV light (5-30ug lysate).

    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-ATF2 (phospho T71 + T53) antibody (ab28812)参考文献

This product has been referenced in:
  • White SJ  et al. Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress. J Cell Physiol 226:2841-8 (2011). WB . Read more (PubMed: 21302282) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (endothelial)
Loading amount 5000 cells
Specification endothelial
Treatment different shear stress exposure for 24 hours
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Mar 05 2010