Anti-ATF2 (phospho T71 + T53)抗体(ab28812)
Key features and details
- Rabbit polyclonal to ATF2 (phospho T71 + T53)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ATF2 (phospho T71 + T53)抗体
参阅全部 ATF2 一抗 -
描述
兔多克隆抗体to ATF2 (phospho T71 + T53) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ATF2 (phospho T53 + T71).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Corresponding non-phospho antibody
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28812于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
组织特异性
Abundant expression seen in the brain. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻译后修饰
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
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别名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
图片
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Ab28812, at a dilution of 1/50, staining ATF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using the ATF2 antibody
Right image: using the same antibody preincubated with synthesized phospho peptide. -
All lanes : Anti-ATF2 (phospho T71 + T53) antibody (ab28812) at 1/1000 dilution
Lane 1 : untreated HeLa cells (5-30ug lysate).
Lane 2 : HeLa cells treated with UV light (5-30ug lysate).
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab28812 被引用在 1 文献中.
- White SJ et al. Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress. J Cell Physiol 226:2841-8 (2011). WB . PubMed: 21302282