概述

  • 产品名称Anti-ATF2 (phospho T71)抗体[E268]
    参阅全部 ATF2 一抗
  • 描述
    兔单克隆抗体[E268] to ATF2 (phospho T71)
  • 特异性ab32019 only detects ATF2 phosphorylated on Threonine 71
  • 经测试应用适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ATF2 aa 50-150.
    Database link: P15336

  • 阳性对照
    • HeLa cells. NIH/3T3 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

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应用

Our Abpromise guarantee covers the use of ab32019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
IP 1/30.
ICC/IF 1/500.
  • 应用说明Is unsuitable for Flow Cyt or IHC.
  • 靶标

    • 功能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
    • 组织特异性Abundant expression seen in the brain.
    • 序列相似性Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • 翻译后修饰Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activating transcription factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      see all

    Anti-ATF2 (phospho T71) antibody [E268] 图像

    • Immunocytochemistry of HeLa (Human epithelial cell line from cervix adenocarcinoma), prepared in FBS free medium overnight labeling ATF2 at 0.9 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/500 . Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counter stain at 2.5 μg/ml. DAPI was used for nuclear counter stain. Confocal image showing the expression was increased on HeLa cells, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30min.

       

       

    • All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.5 µg/ml

      Lane 1 : As above
      Lane 2 : As above

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 54 kDa


      Exposure time : 3 minutes

      Blocking and diluting buffer used was 5% NFDM/TBST

    • All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.1 µg/ml

      Lane 1 : Untreated NIH/3T3 (mouse embryo), prepared in FBS free medium overnight, whole cell lysate
      Lane 2 : NIH/3T3, prepared in FBS free medium overnight, then treated with 10 µg/ml anisomycin for 30 minutes whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 54 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 seconds

      Blocking and diluting buffer used was 2% BSA/TBST.

    • All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 0.1 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma), prepared in FBS free medium overnight, whole cell lysate
      Lane 2 : HeLa, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30 minutes whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 54 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Blocking and diluting buffer used was 2% BSA/TBST .
      The molecular weight is 70kD, consistent with the literature (PMID: 24223142).

    • Lane 1 (input): HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate  10μg.
      Lane 2 (+): HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.
      Lane 3 (-):  Rabbit monoclonal IgG (ab172730) instead of ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.

      Ab32019 Immunoprecipitating ATF2 in Human Hela whole cell lysate. For western blotting ab32019 (1:1000) was used to confirm successful immunoprecipitation. Blocking and diluting buffer used was 5% NFDM/TBST.

    Anti-ATF2 (phospho T71) antibody [E268] (ab32019)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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